Recombinant Antibody Expression Service

Take in your project to the next level – with high-quality recombinant antibody expression services at evitria.

We provide recombinant antibody expression services with our well-established CHO cell platform, ensuring high-quality results from antibody sequences to delivery in as little as 4 weeks.

At evitria, we specialize in recombinant antibody production services using our optimized CHO cell expression platform. This allows us to deliver high-quality antibodies with low endotoxin levels (<1 EU/mg) from your antibody sequences, with turnaround times as short as four weeks.

Our recombinant antibody production services include producing a wide range of antibody types and formats, such as:

other recombinant proteins
Afucosylated antibodies
Fc-silenced antibodies
Bispecific antibodies

LET’S TALK ABOUT YOUR NEEDS

120.000 +

transfections performed since our inception in 2010

99%

projects successfully completed on time

20.000 +

antibodies purified for customers worldwide

4 weeks

from antibody sequence to delivery

Custom recombinant antibody production service – take advantage of more than a decade of experience

With over a decade dedicated to recombinant antibody production, evitria has become a trusted partner for clients worldwide. Since 2010, we have performed over 120,000 transfections and purified more than 20,000 antibodies, serving customers ranging from academic laboratories to global biopharmaceutical companies.

Our expertise encompasses a wide range of expression services, including:

Isotype switching
Chimerization
Glyco-engineering
Bispecific and trispecific antibodies
Fusion antibodies, including Fc fusion proteins

Whether you need a partner to handle your complete antibody production or assistance with specific projects – our team is ready to provide tailored, reliable, and reproducible solutions.

LET’S DISCUSS YOUR PROJECT

Recombinant antibody types produced by evitria

Our recombinant antibody production services cover a diverse array of antibody types and formats to suit your specific requirements. Here is an overview of the different types of specific antibodies that we at evitria can provide you with.

Recombinant Antibodies

Using our CHO cell line expression system, we produce high-quality recombinant monoclonal antibodies that surpass the functionality and reliability of traditional hybridoma-derived and polyclonal antibodies.

Our process offers high throughput, flexible scalability, and is ideal for both discovery research and commercial manufacturing. With low endotoxin levels (<1 EU/mg) and rapid production timelines, we provide full-length antibodies and antibody fragments as a superior in vitro alternative to traditional in vivo methods.

REQUEST YOUR RECOMBINANT ANTIBODY

Afucosylated Antibodies

We offer the production of afucosylated (ADCC-enhanced) antibodies. These antibodies lack fucose residues in their Fc region, leading to enhanced antibody-dependent cellular cytotoxicity. This enhancement increases immunogenicity, thus therapeutic potency, and can reduce side effects.

Through our exclusive licensing partnership with ProBioGen, we utilize their GlymaxX® technology to produce afucosylated antibodies that meet the highest standards.

AFUCOSYLATED ANTIBODY EXPRESSION SERVICE

Fc-Silenced Antibodies

For applications where immune system activation is undesirable, we produce antibodies with reduced or eliminated Fc effector functions.

Utilizing mAbsolve’s STR technology, we generate antibodies with truly silent Fc domains. These antibodies show no binding or activity above background levels across Fc gamma receptors, making them ideal for neutralizing interactions without triggering immune responses.

FC-SILENCED ANTIBODY EXPRESSION SERVICE

Bispecific Antibodies

Our expertise extends to producing bispecific antibodies, capable of simultaneously binding to two different antigens or epitopes. These complex molecules are valuable tools in therapeutic applications, offering targeted approaches in disease treatment.

As we have access to Lonza’s bYlok® technology, we are able to face one of the leading challenges in the bispecific antibody generation process: the correct pairing of heavy chains and light chains.

BISPECIFIC ANTIBODY EXPRESSION SERVICE

Fusion Antibodies and Other Recombinant Proteins

Beyond standard antibody formats, we produce fusion antibodies, including Fc fusion proteins, and other recombinant proteins such as scFvs (single-chain variable fragments), soluble extracellular domains, cytokines, and viral proteins.

Our flexible expression platform allows us to meet a wide range of protein production needs, providing nano-filtered, sterile supernatants suitable for various downstream applications. Furthermore, using CHO cells as our host cells of choice, we can provide not only maximum quality, but also reproducibility of different production processes.

PROTEIN EXPRESSION SERVICE

Unsure on what recombinant antibody you should use?

We’ve got you covered! Regardless of where your research is taking you, Dr. Desmond Schofield is dedicated to supporting you.

Dr. Desmond Schofield
Chief Business Officer at evitria

ds@evitria.com

Recombinant monoclonal antibody production in CHO cells

Our CHO cell-based expression platform allows us to produce recombinant monoclonal antibodies with high purity, exceptional quality, and efficiency. Unlike Escherichia Coli and other bacterial expression systems, CHO cells can even achieve complex human-like post-translational modifications.

Furthermore, by utilizing transient expression of antibody genes in CHO cells, we eliminate the need for laboratory animal immunization, resulting in products free from animal contamination and ethical concerns.

This method also enables us to achieve shorter timelines and higher yields compared to traditional techniques (e.g. hybridoma technology), delivering fully functional antibodies that meet the rigorous demands of modern research and therapeutic applications.

MORE ABOUT THE TECHNOLOGY

From sequence to antibody in 4 weeks

We know how tight timelines in biotechnological projects can be. With resources and know-how at hand, our efficient workflow allows us to provide you with recombinant antibodies within only 4 weeks. Starting with our molecular biology workflow, large-scale expression commences after 2 weeks, followed by purification, extensive analytics, and – finally – shipment to our customers worldwide.

WEEK 1

WEEK 2

WEEK 3

WEEK 4

Pilot Study*

Larger-scale expression

Purification

Finaly analytics

Shipment

FAQs on recombinant antibody production

Is evitria using laboratory animals for recombinant antibody production?

No, nor do we use E.Coli or HEK cells. Instead, we use mammalian cell cultures. We prefer Chinese Hamster Ovarian (CHO) cells in our mammalian expression system, since that comes with numerous advantages over the use of laboratory animals.

Why is transient expression in CHO beneficial?

Recombinant antibody expression in CHO allows large-scale recombinant antibody production with low levels of endotoxin (<1 EU/mg). Moreover, CHO cell antibody production is much faster than any traditional technique. We are up to months faster than antibody suppliers relying on hybridoma cells.

What does mammalian expression mean?

Mammalian expression is the process of biological protein production in mammals or mammalian cells, such as HEK293 or CHO cells. Some proteins, such as antibodies, can’t be produced in fully functional form in other cell cultures.

What is the difference between an antibody supplier or an antibody vendor and the recombinant antibody expression service at evitria?

evitria offers custom antibodies to their customers. Our service includes recombinant antibodies, afucosylated antibodies or other proteins. In contrast to a conventional antibody supplier, we do not offer a catalog with standardized antibodies.

What is antibody engineering?

Antibody engineering is the process of changing and optimizing antibodies to equip them with better or novel properties. In this case, the fab fragment is modified. One example is the fine-tuning of antigen binding sites on their variable regions to enhance affinity or specificity towards an antigen.

Another example is the fusion of two antibodies with distinct antigens, to form a bispecific antibody that shows affinity towards both antigens.

How do you purify your antibodies?

For the removal of residing reagents, amino acids and other byproducts, protein A purification is standard in biotechnology, and we use alternative resins such as Protein G, L, Kappa/Lambda-Select or anti-IgG-CH1 as needed. An additional size exclusion chromatography (SEC) purification step can be added, as well as ion exchange chromatography. Please inquire for more details.

Read more on recombinant antibody purification

What is the average turnaround time in antibody production?

Our average timeline is between 4 and 6 weeks for new antibodies. If we have produced the antibody before then reproduction will take 2.5-3.5 weeks.

Can you produce bispecific or trispecific antibodies?

We have produced both bispecific and trispecific antibodies in various formats. Please contact your local evitria representative to discuss your specific project.

Do you offer any downstream assays?

No, but please see our “Partners” page to find best-in-class solutions for required services.

Do you offer characterization like conjugation or post-purification modifications?

No, but please see our “Partners” page to find best-in-class solutions for required services. We work together with established partners for optimization of mAb and rAb development workflows.

Can you send us your vectors?

We are unable to send our proprietary expression vectors but can clone into a freedom-to-use vector and send for an additional fee.

What recombinant antibody formats are there?

There are various different formats of immunoglobulins, e.g. full-length IgG1, VHH, bispecific or chimeric abs.

What is a recombinant gene?

A recombinant gene is a sequence of genetic code that is inserted into different genetic material by recombinant DNA technology. Recombinant gene sequences (previously undergone the process of gene synthesis) are used to transfer blueprints of enzymes and proteins (e. g. recombinant antibodies) into cells in order to have them produce said protein (e.g. for recombinant antibody technology).

Do you use stable or transient transfection for antibody production?

At evitria, we focus on transient antibody production. Thereby, we rely on CHO cells as our expression system of choice, as they come with several advantages in terms of quality and speed.

Does evitria also take over antibody sequencing services?

No, evitria works with partners to ensure maximum value to customers. For sequencing issues in the production of recombinant antibodies, Rapid Novor is evitria’s partner.

What is phage display technology?

Antibody phage display technology is a method used to study protein interactions, particularly in antibody development. It involves inserting genes encoding antibodies into plasmids, which are then introduced into bacteriophages. This process allows billions of antibodies to be presented on the surface of the bacteriophage. The resulting antibody library enables the selection of antibodies with high affinity for specific targets (e.g. with ELISA testing or western blotting), aiding in therapeutic antibody discovery and development.