Accelerate your therapeutic research with our high-quality afucosylated antibody expression service at evitria.
We provide afucosylated antibody expression services based on CHO cells. This enables us to express high-quality afucosylated antibodies for projects at different stages and scales – with no amino acid alterations.
Our expertise ensures that you receive afucosylated antibodies with enhanced Antibody-Dependent Cellular Cytotoxicity (ADCC), crucial for advancing therapeutic antibodies in immunotherapy and many other applications.
We can achieve this by accessing ProBioGen’s proprietary GlymaxX® system, ensuring the absence of fucose residues without the need for altering the amino acid sequence of your antibody.1
GlymaxX® antibodies purified for customers worldwide
4 weeks
from antibody sequence to delivery
Rapid Afucosylated antibody production service to support your development
There are many ways to enhance ADCC by Fc engineering (Table 1), but these require changing the amino acid sequence of your antibody – a big risk in any therapeutic development project.
Afucosylation, i.e. the removal of core fucose residues from antibody glycan structures is a way of enhancing ADCC without any amino acid substitutions. At evitria, we combine ProBioGen’s proprietary cell engineering approach, GlymaxX® with our transient CHO antibody expression platform to rapidly deliver high quality afucosylated antibodies.
By increasing ADCC through afucosylation, your antibody drugs can achieve greater efficacy against target cells. This is achieved by enhancing the interaction between the antibody’s Fc region and FcγrIIIa receptors on effector cells like natural killer cells (NK cells) or T-cells, leading to improved antibody-dependent cell-mediated cytotoxicity.2
Expression service for truly afucosylated antibodies? No matter of course!
ProBioGen’s GlymaxX® technology seamless integrates with our rapid transient CHO expression service. With these technologies, we can produce your ideal side-by-side comparison to study the impact of antibody afucosylation.
Simulataneously, we transfect one CHO cell culture with your recombinant monoclonal antibody, and another culture with your antibody plus the RDB enzyme of GlymaxX®. This enzyme redirects the fucose synthesis pathways within our CHO cells, and so prevents the addition of core fucose onto the Asn298 glycan structures within the antibody Fc domain. Both cultures grow, express the transfected antibody, and are purified in parallel using evitria’s robust plaform, delivering high quality material and the ideal wild-type control.
GlymaxX® avoids the risks associated with Fc mutations that could have an impact on immunogenicity, developability, and stability. By avoiding changes to the antibody’s oligosaccharide structures or N-linked glycosylation sites, we maintain the natural effector functions and stability of the antibody. We have verified this through mass spectrometry analysis, the gold standard of antibody characterisation, and can offer this enhanced analytics verification to any recombinant antibody production we perform.
We focus exclusively on recombinant antibody production through transient expression in CHO cells. This means that all our strength and expertise go into the production of mAbs, bsAbs, and other antibody-related formats – including afucosylated antibodies, Fc-silenced antibodies, and other proteins. We also offer bispecific antibody expression services. Our dedicated team ensures rapid turnaround times and high-quality deliverables, so you can progress your research without delay.
Long-lasting, healthy customer relationships are very
important to us. Anonymity is therefore part of our corporate DNA.
“We have been working with evitria for several years and highly appreciate the high consistency of the delivered material.”
Sr. Scientist, Biotherapeutics, Switzerland
“We have been working with evitria since 2022. We appreciate the openness and flexibility of the team, the high speed and quality of the proteins they deliver.”
Radiopharm Theranostics Ltd.
“We are happy paying for the assured quality that evitria brings.”
Sr Scientist, Biotherapeutics, Series B Startup, Boston
Unsure on what afucosylated antibody you should use?
We’ve got you covered! Regardless of where your research is taking you, we are dedicated to supporting you.
Our partnership with ProBioGen allows us to access the GlymaxX® platform – a cutting-edge technology for afucosylation. This system inhibits the enzymatic addition of fucose to the N-glycan structures on the Fc region of antibodies without any DNA sequence modifications. In other words: While alternative ADCC-enhancement approaches rely on Fc mutations that introduce risk of immunogenicity, developability, and stability risks, we can provide ADCC-enhanced materail without changing the sequence of your antibody.
By utilizing CHO host cells for our mammalian expression platform, we ensure high yields and scalability suitable for both small-scale assays and larger therapeutic antibody production. Our transient expression approach eliminates the need for stable cell line development, saving you time and resources.
With GlymaxX®, you receive antibodies with improved effector functions without compromising on quality or yield.
We know how tight timelines in biotechnological projects can be. With resources and know-how at hand, our efficient workflow allows us to provide you with recombinant antibodies within only 4 weeks. Starting with a pilot study, large-scale expression commences after 2 weeks, followed by purification, extensive analytics, and – finally – shipment of the deliverables to our customers worldwide.
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High-Throughput Antibody Production Service
Accelerate early-stage antibody discovery with parallel CHO expression of up to several hundred constructs per project.
evitria’s HTP antibody production service delivers purified, assay-ready antibodies within 4 weeks – with the same Swiss-quality analytical package used at clinical scale. No host-cell transitions, no re-optimization, no delays.
Due to the Corona pandemic, the term “antibody” has become one of the buzzwords of 2020/2021. The history of antibodies, also known as immunoglobulin, of course reaches back far further than the early months of 2020 – their meaning and importance has not only started gaining relevance with the outbreak of COVID-19.
The increasing demand of therapeutic antibodies can lead to capacity issues in your antibody production. Learn how to avoid bottlenecks and why it makes sense to outsource your antibody expression.
Therapeutic antibodies can be split into two broad families: those which enhance cell killing by activation of the host’s immune system, and those which block an interaction or neutralize a pathway. Read more about the benefits of sourcing STR-silenced antibodies through evitria!
evitria expresses afucosylated antibodies using ProBioGen’s GlymaxX® platform, a cutting-edge technology that inhibits the addition of fucose to the N-glycan structures on the Fc region of antibodies. This process is done without any alteration of the antibody sequence, ensuring the antibodies retain their natural structure and functionality.
ProBioGen’s GlymaxX® platform offers several key benefits for the production of afucosylated antibodies:
Liu, Scot, et al. “Afucosylated Antibodies Increase Activation of FcγRIIIa-Dependent Signaling Components to Intensify Processes Promoting ADCC.” Cancer Immunology Research, vol. 3, no. 2, 2015, pp. 173-183. https://doi.org/10.1158/2326-6066.CIR-14-0125. ↩︎
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